The former NBC sitcomA.P. Biohas a robust cast full of hilarious main and supporting characters.Mike O’Brien ofSaturday Night Liveacclaim created the original sitcom, which first premiered on July 25, 2025. The show is centered around professor turned high-school teacher Jack Griffin, who is played byGlenn Howerton ofIt’s Always Sunny in Philadelphia.A.P. Biofollows Jack as he transitions from being a philosophy professor at Harvard University to an A.P. Biology teacher at his hometown high school after falling from grace due to a YouTube video.
A.P. Bioran for two seasons on NBC before moving to Peacockfor its final two seasons.The series was canceled twiceeven though itmaintained an impressive Rotten Tomatoesscore of 88%, with its second and third seasons earning perfect 100% critic scores. Despite the positive reviews of A.P. Bio, there was not enough consistent viewership for the series to continue past four seasons. Over its four-year span,A.P. Biofeatured several notable guest stars and cameo appearances, making it one of the most unappreciated sitcoms in recent memory.
A.P. Bioseasons 1-4 are available to stream on Netflix and Peacock.
Glenn Howerton as Jack Griffin
Actor: Glenn Howerton, 48, is an American actor who was born in Japan. He is best known for playing Dennis Reynolds on the hit FX comedy seriesIt’s Always Sunny In Philadelphia,which is the longest-running original comedy series in television history. Howeton co-created the series with co-star Rob McElhenney. Howerton made his film debut with 2005’sMust Love Dogsand has also appeared in films such asCrank,The Strangers,Everything Must Go,The Hunt, andBlackberry. Howerton’s other notable performances in television includeThat ’80s Show(2002),The Mindy Project(2012–2017),Fargo(2014), andVelma(2023-2024).
Blackberry
Jim Balsillie
Character: Howerton stars as the main character Dr. Jack Griffin inA.P. Bio.
Lyric Lewis as Stef Duncan
Actor: Lyric Lewis, 39, is an American actress and writer from New Orleans, Louisiana. Lewis is best known forWaffles + Mochi,Mad TV, and the Dreamworks seriesCurses!on Apple TV+. She has made guest appearances on several notable comedy series and sitcoms, including but not limited toBrooklyn Nine-Nine,Baskets,iCarly,Party Down, andNight Court. She has also made several appearances as herself on television shows such asDrunk History,Is It Cake?, andThe Wheel. Lewis is expected to star in the upcoming short filmI Am a Gigantosaurus, Actuallyalongside Alison Brie.
Baskets
Monica
Character: Lewis stars as the history teacher Stef Duncan inA.P. Bio.
Mary Sohn as Mary Wagner
Actor: Mary Sohn, 44, is an American actress from Illinois. Sohn is best known for her roles inThe Boss,Between Two Ferns: The Movie,Love,Grace and Frankie, andComedy Bang! Bang!. She recently appeared in an episode ofCurb Your Enthusiasmand in the TBS seriesMiracle Workers. She rose to prominence after guest starring in an episode of the notable HBO comedy seriesHello Ladiesin 2013. She has also guest starred in episodes ofTeachers,Superior Donuts,Shrink, andBajillion Dollar Propertie$.
Character: Sohn plays the art teacher Mary Wagner inA.P. Bio.
Jean Villepique as Michelle Jones
Actor: Jean Villepique is an American actress from New Jersey who attended Northwestern University. As a former member of The Second City, she is best known for her roles inBoJack HorsemanandUp All Night. She made her television debut in 2007 in episodes of30 RockandCurb Your Enthusiasm. She also starred alongside Jason Segel and Paul Rudd in the 2009 comedyI Love You, Man. She has guest starred in many notable comedy series such asModern Family,Workaholics,Grey’s Anatomy,Baskets,New Girl, andBetter Call Saul. She is also known for playing Gretchen inSharp Objects.
Character: Villepique plays home ec teacher Michelle Jones inA.P. Bio.
Paula Pell as Helen Henry Demarcus
Actor: Paula Pell, 61, is an American comedy writer, producer, and actress from Illinois. Pell is a prolific writer forSaturday Night Livewho has writing credits for more than 315SNLepisodes between 1995 and the present day. As an actress, Pell has appeared inParks and Recreation,30 Rock,Anchorman 2,Inside Out,The Mindy Project,Unbreakable Kimmy Schmidt, andInside Out 2. She also voiced various roles in the Netflix animated seriesBig Mouth. She is slated to star alongside Matt Berry, Bill Hader, and Quinta Bronson in the upcomingThe Cat in the Hatfilm as well asSummer of 69with Chloe Fineman, Charlie Day, and Natalie Morales.
Character: Pell plays school administrator Helen Henry Demarcus inA.P. Bio.
Patton Oswalt as Principal Ralph Durbin
Actor: Patton Oswalt, 55, is an American comedian and actor from Virginia who has more than 250 professional acting credits to his name. He is best known for his roles inRatatouille,Young Adult,The Secret Life of Walter Mitty, andSeeking a Friend for the End of the World. He has recently appeared inHacks,Manhunt,Ghostbusters: Frozen Empire,Family Guy,What We Do in the Shadows, and80 for Brady. He is also an award-winning stand-up comedian who has been nominated for 4 Emmys with one win for Outstanding Writing for a Variety Special for 2016’sTalking for Clapping.
A.P. Bio Supporting Cast & Characters
Aparna Brielle as Sarika: Brielle is best known forFUBARandMech Cadets.
Nick Peine as Marcus: Peine is best known forOffice Christmas PartyandJust Getting Started.
Allisyn Snyder as Heather: Snyder is best known forAstrid CloverandMeet Dave.
Eddie Leavy as Anthony: Leavy is best known forBest. World. Weekend. Ever.andThose Who Can’t.
Jacob Houston as Victor: Houston is best known forYellowjacketsandL.A.’s Finest.
Sari Arambulo as Grace: Arambulo is best known forAlexa & KatieandParty of Five.
Spence Moore II as Dan Decker: Moore is best known forCreed IIIandWe Are Who We Are.
Marisa Baram as Marissa: Baram is best known forHenry DangerandReal Husbands of Hollywood.
Miguel Lee as Eduardo: Lee is best known forDaveandThe Saver.
Charlie McCrackin as Coach Dick Novak: McCracking is best known forIntergalactic RadioandUnstable.
A.P. Bio Cameo Guide
Nikki Glaser as Chloe from Baltimore: Glaser is best known forTrainwreckandThe Roast of Tom Brady.
Niecy Nash as Kim Burke: Nash is best known forReno 911!andGuess Who.
Taran Killam as Mr. Vining: Killam is best known forSaturday Night Liveand12 Years a Slave.
Mark Proksch as Philip: Proksch is best known forBetter Call SaulandWhat We Do in the Shadows.
Christopher Lloyd as Melvin: Lloyd is best known forBack to the FutureandThe Addams Family.
Jon Lovitz as Robin Schwonk: Lovitz is best known forA League of Their OwnandRat Race.
Ron Funches as Hans: Funches is best known forTrollsandInside Out 2.
Angela Kinsey as Brenda: Kinsey is best known forThe Officeand has a cameo inA.P. Bio.
A.P. Bio
Cast
A.P. Bio is an NBC comedy that premiered in 2018. The sitcom stars It’s Always Sunny in Philidelphia’s Glenn Howerton as Jack Griffin, a former philosophy professor who starts working as a high school advanced placement biology teacher. After season 2, A.P. Bio moved to Peacock for two seasons before getting canceled in 2021.
